三花假卫矛中的一个新三萜化合物

丁佩, 周蔓青, 王奎武

中国药学杂志 ›› 2016, Vol. 51 ›› Issue (18) : 1557-1561.

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中国药学杂志 ›› 2016, Vol. 51 ›› Issue (18) : 1557-1561. DOI: 10.11669/cpj.2016.18.004
论著

三花假卫矛中的一个新三萜化合物

  • 丁佩, 周蔓青, 王奎武*
作者信息 +

A New Cytotoxic Triterpenoid from Microtropis triflora Merr. et Freem.

  • DING Pei, ZHOU Man-qing, WANG Kui-wu*
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摘要

目的 研究三花假卫矛(Microtropis triflora Merr. et Freem.) 的化学成分。方法 采用硅胶柱色谱,Sephadex LH-20凝胶柱色谱,半制备高效液相色谱仪等分离技术进行分离纯化,通过理化性质和波谱方法鉴定化合物的结构。结果 从三花假卫矛的甲醇提取物中分离得到10个化合物。其结构分别鉴定为:3,25-环氧-1α,3α,11α,12,23,24-六羟基-乌苏-12-烯(1),β-香树脂醇(2),β-香树脂醇棕榈酸酯(3),3β-羟基-12-齐墩果烯-11-酮-3-棕榈酸酯(4),羽扇豆醇(5),木栓酮(6),3-氧-木栓烷-28-羧酸(7),齐墩果酸(8),大子五层龙酸(9)和直楔草酸(10)。结论 化合物1为一个新的三萜类化合物,并对Bcap37及SMMC7721肿瘤细胞具有显著抑制作用,IC50值分别为 27.86和11.38 μg·mL-1

Abstract

OBJECTIVE To study the chemical constituents of Microtropis triflora Merr. et Freem. METHODS The compounds were isolated and purified by various chromatographic technigues such as silica gel, Sephadex LH-20, and pre-HPLC. Their structures were identified on the basis of chemical properties and spectral analysis. RESULTS Ten triterpenes were isolated and elucidated as 3, 25-epoxy-1α, 3α, 11α, 12, 23, 25-hexahydroxy-urs-12-en(1), β-amyrin(2), β-amyrin palmitate(3), 3β-hydroxy-11-oxo-olean-12-enyl-3-palmitate(4), lupeol(5), friedelin(6), 3-oxo-28-friedelanoic acid(7), oleanolic acid(8), salaspermic acid(9), and orthosphenic acid(10). CONCLUSION Compound 1 is a new compound and has cytotoxic activity against Bcap37 and SMMC7721 cells with IC50 values of 27.86 and 11.38 μg·mL-1, respectively.

关键词

卫矛科 / 三花假卫矛 / 化学成分 / 三萜 / 肿瘤细胞毒活性 / 3 / 25-环氧-1α / / 11α / 12 / 23 / 24-六羟基-乌苏-12-烯

Key words

Celastraceae / Microtropis triflora / chemical constituent / triterpene / tumor cytotoxic acivity / 3, 25-epoxy-1α, 3α, 11α, 12, 23, 25-hexahydroxy-urs-12-en

引用本文

导出引用
丁佩, 周蔓青, 王奎武. 三花假卫矛中的一个新三萜化合物[J]. 中国药学杂志, 2016, 51(18): 1557-1561 https://doi.org/10.11669/cpj.2016.18.004
DING Pei, ZHOU Man-qing, WANG Kui-wu. A New Cytotoxic Triterpenoid from Microtropis triflora Merr. et Freem.[J]. Chinese Pharmaceutical Journal, 2016, 51(18): 1557-1561 https://doi.org/10.11669/cpj.2016.18.004
中图分类号: R284   

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